Description:

SureBond-XF is a xeno-free coating solution optimized for long-term or low density culture of human iPSC-derived neural stem cells and neurons

Axol SureBond-XF provides a defined, xeno-free surface specifically designed to promote optimal growth and attachment of human neural progenitor cells and neurons. After coating, and a short 4-hour incubation period (at 37^oC), your cell culture flask or plate will be ready to receive your neural progenitor cells or neurons allowing you to get on with your research and saving your valuable time.

Axol Human iPSC-Derived Neural Stem Cells (ax0015) were expanded on vessels coated with SureBond-XF and passaged using Unlock-XF (ax0044-XF). Post-passage the neural progenitors were left to spontaneously differentiate in Neural Maintenance-XF Medium (ax0032-500).

SureBond-XF supports low density cultures

Human Neural Stem Cells (ax0016) were plated onto SureBond-XF coated culture vessels at low density. The cells were stained for Tuj1 expression and counterstained using DAPI. 

Human Neural Progenitors Cultured on Sure Bond-XF form neural rosettes

Human Neural Stem Cells (ax0015) cultured on SureBond-XF coated culture vessels (Nestin Foxg1 DAPI).

Human Neural Progenitors Cultured on SureBond-XF express neural progenitor markers

Human Neural Stem Cells (ax0015) cultured on SureBond-XF coated culture vessels express Pax6 and Nestin. 

Advantages

• Xeno-free coating solution

• Short coating procedure (4 hours)

• Supports low density cultures

Specifications

Catalog Number: ax0041XF

Concentration: 200X

Format: Liquid

Volume: 1 mL

Sterility: Sterile

Shipping Condition: Blue Ice

Storage: Store at 4ºC and use within 6 months

Applications

• Coating cell culture vessels for human neural progenitor cells and cerebral cortical neurons

Product Specification: