*Pleasenotetheproductcodewillbechangingfromax0041XFtoax0053.
Description:
SureBond-XFisaxeno-freecoatingsolutionoptimizedforlong-termorlowdensitycultureofhumaniPSC-derivedneuralstemcellsandneurons
AxolSureBond-XFprovidesadefined,xeno-freesurfacespecificallydesignedtopromoteoptimalgrowthandattachmentofhumanneuralProgenitorcellsandneurons.Aftercoating,andashort4-hourincubationperiod(at37oC),yourcellcultureflaskorplatewillbereadytoreceiveyourneuralprogenitorcellsorneuronsallowingyoutogetonwithyourresearchandsavingyourvaluabletime.
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AxolHumaniPSC-DerivedNeuralStemCells(ax0015)wereexpandedonvesselscoatedwithSureBond-XFandpassagedusingUnlock-XF(ax0044-XF).Post-passagetheneuralprogenitorswerelefttospontaneouslydifferentiateinNeuralMaintenance-XFMedium (ax0032-500).
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SureBond-XFsupportslowdensitycultures
HumanNeuralStemCells(ax0016)wereplatedontoSureBond-XFcoatedculturevesselsatlowdensity.ThecellswerestainedforTuj1expressionandcounterstainedusingDAPI.
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HumanNeuralProgenitorsCulturedon SureBond-XFformneuralrosettes
HumanNeuralStemCells(ax0015)culturedon SureBond-XF coatedculturevessels(Nestin Foxg1 DAPI).
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HumanNeuralProgenitorsCulturedonSureBond-XFexpressneuralprogenitorMarkers
HumanNeuralStemCells(ax0015)culturedonSureBond-XFcoatedculturevesselsexpressPax6andNestin.