Description:
Next-generationneuralcellculturemediumrestrictedtoanimal-freecomponents.Ithasbeenoptimizedtosupportlowdensitycultureandlong-termfunctionalmaturation.
Xeno-FreeCellCultureMediumforoptimalmaintenanceof
humanneuralstemcellsandneuralcelltypes
Forlong-termneuronalcultures,Axol"s NeuralMaintenance-XFMedium providesanidealenvironmenttosupportaxonalelongationandsynapticactivity.NeuralStemCellsculturedinthismediummaintainthecapacitytodifferentiateintoneuronalandgliallineages.Themediumiscompletelydefinedensuringlot-to-lotconsistencyandreliableperformancebetweenexperiments.NeuralMaintenance-XFMediumcanmaintainhealthyneuronswhenculturedatlowdensity(10,000cells/cm2).
NeuralStemCells wereculturedona SureBond+ReadySet coatedculturevesselusing NeuralDifferentiation-XFMedium. 54hafterthawing,theNSCscellswereswitched to NeuralMaintenance-XFMedium.Theresultingneuronswerestainedfor TUJ1 expressionandcounterstainedusing DAPI. ImagetakenatDay14.
NeuralStemCells wereculturedona SureBond+ReadySet coatedculturevesselusing NeuralDifferentiation-XFMedium. 54hafterthawing,theneuralstemcellswereswitched to NeuralMaintenance-XFMedium. TheresultingneuronswerestainedusinganantibodytargetingMAP2.ThecellswerecounterstainedusingDAPI.ImagetakenatDay14.
NeuralStemCells werebrieflyexpandedinNeuralExpansion-XFMedium andpassagedontoa SureBond+ReadySet coatedculturevessel.Thecellsweremaintainedin NeuralMaintenance-XFMedium.Thecellswerecounterstainedusing DAPI.Neuralstemcellscanbevisualizedas Ki67-positivecells.NeuronsdifferentiatedfromtheneuralstemcellsareshownusingtheMarker TUJ1.ImagetakenatDay7.
ProductSpecification:
Size | 500mL |
Shippingconditions | Dryice |
Storageconditions | -80°C |
Xenofree | Yes |