Description:
NeuralStemCellsfromaTrisomyXPatient
TrisomyX(TripleX)syndromeoccursatafrequencyofapproximately1in1000newbornfemales.Thesyndromeisassociatedwithanincreasedriskofseizuresandmayaffectshort-termmemoryandlearningalthoughthemajorityofwomenaffectedshowfewclinicalsymptoms.AxolhasdevelopediPSC-derivedneuralstemcellsfromapatientwiththetripleXchromosomalabnormality.AlloftheiPScellsexamined(20individualcellskaryotyped),hadtrisomyX.
AxolHumaniPSC-DerivedNeuralStemCells
AxolHumaniPSC-DerivedNeuralStemCells(NSCs)arederivedfromintegration-free,inducedpluripotentstemcells(iPSCs)underfullydefinedneuralinductionconditions.TheNSCsexpresstypicalMarkersofcerebralcorticalneuralstemandProgenitorcellssuchasPAX6,FOXG1andnestin,andspontaneouslyformpolarizedneuraltube-likerosettestructureswhenplatedasamonolayerinculture(seebelow).Additionally,AxolNSCsarecapableofgeneratingaspectrumofcerebralcorticalexcitatoryandinhibitoryneuronsthatareelectricallyactiveandhavetheABIlitytoformfunctionalsynapsesandcircuitsinvitro.Afterthawingandplating,theneuralstemcellsterminallydifferentiatetoacquirematureelectrophysiologicalproperties,andformfunctionalsynapticnetworksoveraperiodof40~50days.
AxolNSCsareeasytodifferentiatetoneuronsormixedneuralcelltypes,followingourprotocolsandusingourtailoredmediaandreagentbundles.AhighlypurepopulationofneuronscanbegeneratedfromAxolNSCsfollowingthesynchronousdifferentiationprotocol.Usingourspecializedcoatingreagents,neuronsderivedfromAxolNSCscanbemaintainedinculturelong-term(>1year).NSCsareavailablefrommultipledonorstosuityourresearchneedsandhavebeencharacterizedextensively.

ProductSpecification:
| Startingmaterial | Dermalfibroblast | 
| Donorgender | Female | 
| Donorageatsampling | 74yrs | 
| Karyotype | TrisomyX(47,XXX) | 
| Reprogrammingmethod | Episomalvector | 
| Inductionmethod | Monolayer&chemicallydefinedmedium | 
| Geneticmodification | None | 
| Size | ≥1.5millioncells | 
| Celltype | iPSC-derivedneuralstemcells | 
| Growthproperties | Adherent | 
| Shippingconditions | Dryice | 
| Storageconditions | Liquidnitrogen | 

		