Description:
iPSC-DerivedNeuralStemCellsforEpilepsyStudies
Usingourexpertiseinreprogrammingandneuralinduction,AxolhasdifferentiatedneuralstemcellsfromiPSCsderivedfromapatientthatwasclinicallydiagnosedwithepilepsy.OurlatestblogdiscussestheneedforhumaniPSC-derivedneuralcellmodelsfromhealthyandpatientdonorstohelpfurtherourunderstandingofneuronalnetworksandidentifyeffectivetreatmentsforconditionssuchasepilepsy.
AxolHumaniPSC-DerivedNeuralStemCells
AxolHumaniPSC-DerivedNeuralStemCells(NSCs)arederivedfromintegration-free,inducedpluripotentstemcells(iPSCs)underfullydefinedneuralinductionconditions.TheNSCsexpresstypicalMarkersofcerebralcorticalneuralstemandProgenitorcellssuchasPAX6,FOXG1andnestin,andspontaneouslyformpolarizedneuraltube-likerosettestructureswhenplatedasamonolayerinculture(seebelow).Additionally,AxolNSCsarecapableofgeneratingaspectrumofcerebralcorticalexcitatoryandinhibitoryneuronsthatareelectricallyactiveandhavetheABIlitytoformfunctionalsynapsesandcircuitsinvitro.Afterthawingandplating,theneuralstemcellsterminallydifferentiatetoacquirematureelectrophysiologicalproperties,andformfunctionalsynapticnetworksoveraperiodof40~50days.AxolNSCsareeasytodifferentiatetoneuronsormixedneuralcelltypes,followingourprotocolsandusingourtailoredmediaandreagentbundles.AhighlypurepopulationofneuronscanbegeneratedfromAxolNSCsfollowingthesynchronousdifferentiationprotocol.Usingourspecializedcoatingreagents,neuronsderivedfromAxolNSCscanbemaintainedinculturelong-term(>1year).NSCsareavailablefrommultipledonorstosuityourresearchneedsandhavebeencharacterizedextensively.

ProductSpecification:
| Startingmaterial | Dermalfibroblast |
| Donorgender | Female |
| Donorageatsampling | 5months |
| Donorageofonset | inutero |
| Reprogrammingmethod | Episomalvector |
| Inductionmethod | Monolayer&chemicallydefinedmedium |
| Geneticmodification | None |
| Size | ≥1.5millioncells |
| Kitcomponents | 1vialofNeuralStemCells(≥1.5millioncells)and1bottleofNeuralPlating-XFMedium(30mL) |
| Celltype | iPSC-derivedneuralstemcells |
| Growthproperties | Adherent |
| Diseaseinformation | Thepatientpresentedwithprenatal(3-4weeksbeforedelivery)tremorsinutero.Afterbirth,thepatientexhibitedbradycardia,abnormalEEG(centralapneaspresent)andearlymyoclonicencephalopathy.Thepatient"sepilepsywascharacterizedfurtherby:notederraticseizures(1to5perday),poororalandmotorskills,inabilitytofollowwithgazeandthebrainstemauditoryevokedresponse(BAER)revealedonlywaveformsIandIIIsuggestiveofupperpontinedysfunction.ThedonorwastreatedwithphenobarbitalandKlonopin. |
| Shippingconditions | Dryice |
| Storageconditions | Liquidnitrogen |

