Description:
iPSC-DerivedNeuralStemCellsforEpilepsyStudies
Usingourexpertiseinreprogrammingandneuralinduction,AxolhasdifferentiatedneuralstemcellsfromiPSCsderivedfromapatientthatwasclinicallydiagnosedwithepilepsy.OurlatestblogdiscussestheneedforhumaniPSC-derivedneuralcellmodelsfromhealthyandpatientdonorstohelpfurtherourunderstandingofneuronalnetworksandidentifyeffectivetreatmentsforconditionssuchasepilepsy.
AxolHumaniPSC-DerivedNeuralStemCells
AxolHumaniPSC-DerivedNeuralStemCells(NSCs)arederivedfromintegration-free,inducedpluripotentstemcells(iPSCs)underfullydefinedneuralinductionconditions.TheNSCsexpresstypicalMarkersofcerebralcorticalneuralstemandProgenitorcellssuchasPAX6,FOXG1andnestin,andspontaneouslyformpolarizedneuraltube-likerosettestructureswhenplatedasamonolayerinculture(seebelow).Additionally,AxolNSCsarecapableofgeneratingaspectrumofcerebralcorticalexcitatoryandinhibitoryneuronsthatareelectricallyactiveandhavetheABIlitytoformfunctionalsynapsesandcircuitsinvitro.Afterthawingandplating,theneuralstemcellsterminallydifferentiatetoacquirematureelectrophysiologicalproperties,andformfunctionalsynapticnetworksoveraperiodof40~50days.AxolNSCsareeasytodifferentiatetoneuronsormixedneuralcelltypes,followingourprotocolsandusingourtailoredmediaandreagentbundles.AhighlypurepopulationofneuronscanbegeneratedfromAxolNSCsfollowingthesynchronousdifferentiationprotocol.Usingourspecializedcoatingreagents,neuronsderivedfromAxolNSCscanbemaintainedinculturelong-term(>1year).NSCsareavailablefrommultipledonorstosuityourresearchneedsandhavebeencharacterizedextensively.
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ProductSpecification:
Startingmaterial | Dermalfibroblast |
Donorgender | Female |
Donorageatsampling | 5months |
Donorageofonset | inutero |
Reprogrammingmethod | Episomalvector |
Inductionmethod | Monolayer&chemicallydefinedmedium |
Geneticmodification | None |
Size | ≥1.5millioncells |
Kitcomponents | 1vialofNeuralStemCells(≥1.5millioncells)and1bottleofNeuralPlating-XFMedium(30mL) |
Celltype | iPSC-derivedneuralstemcells |
Growthproperties | Adherent |
Diseaseinformation | Thepatientpresentedwithprenatal(3-4weeksbeforedelivery)tremorsinutero.Afterbirth,thepatientexhibitedbradycardia,abnormalEEG(centralapneaspresent)andearlymyoclonicencephalopathy.Thepatient"sepilepsywascharacterizedfurtherby:notederraticseizures(1to5perday),poororalandmotorskills,inabilitytofollowwithgazeandthebrainstemauditoryevokedresponse(BAER)revealedonlywaveformsIandIIIsuggestiveofupperpontinedysfunction.ThedonorwastreatedwithphenobarbitalandKlonopin. |
Shippingconditions | Dryice |
Storageconditions | Liquidnitrogen |