Description:
iPSC-DerivedNeuralStemCellsforEpilepsyStudies
Usingourexpertiseinreprogrammingandneuralinduction,AxolhasdifferentiatedneuralstemcellsfromiPSCsderivedfromapatientthatwasclinicallydiagnosedwithepilepsy.OurlatestblogdiscussestheneedforhumaniPSC-derivedneuralcellmodelsfromhealthyandpatientdonorstohelpfurtherourunderstandingofneuronalnetworksandidentifyeffectivetreatmentsforconditionssuchasepilepsy.
AxolHumaniPSC-DerivedNeuralStemCells
AxolHumaniPSC-DerivedNeuralStemCells(NSCs)arederivedfromintegration-free,inducedpluripotentstemcells(iPSCs)underfullydefinedneuralinductionconditions.TheNSCsexpresstypicalMarkersofcerebralcorticalneuralstemandProgenitorcellssuchasPAX6,FOXG1andnestin,andspontaneouslyformpolarizedneuraltube-likerosettestructureswhenplatedasamonolayerinculture(seebelow).Additionally,AxolNSCsarecapableofgeneratingaspectrumofcerebralcorticalexcitatoryandinhibitoryneuronsthatareelectricallyactiveandhavetheABIlitytoformfunctionalsynapsesandcircuitsinvitro.Afterthawingandplating,theneuralstemcellsterminallydifferentiatetoacquirematureelectrophysiologicalproperties,andformfunctionalsynapticnetworksoveraperiodof40~50days.AxolNSCsareeasytodifferentiatetoneuronsormixedneuralcelltypes,followingourprotocolsandusingourtailoredmediaandreagentbundles.AhighlypurepopulationofneuronscanbegeneratedfromAxolNSCsfollowingthesynchronousdifferentiationprotocol.Usingourspecializedcoatingreagents,neuronsderivedfromAxolNSCscanbemaintainedinculturelong-term(>1year).NSCsareavailablefrommultipledonorstosuityourresearchneedsandhavebeencharacterizedextensively.

ProductSpecification:
| Startingmaterial | Dermalfibroblast | 
| Donorgender | Female | 
| Donorageatsampling | 5months | 
| Donorageofonset | inutero | 
| Reprogrammingmethod | Episomalvector | 
| Inductionmethod | Monolayer&chemicallydefinedmedium | 
| Geneticmodification | None | 
| Size | ≥1.5millioncells | 
| Kitcomponents | 1vialofNeuralStemCells(≥1.5millioncells)and1bottleofNeuralPlating-XFMedium(30mL) | 
| Celltype | iPSC-derivedneuralstemcells | 
| Growthproperties | Adherent | 
| Diseaseinformation | Thepatientpresentedwithprenatal(3-4weeksbeforedelivery)tremorsinutero.Afterbirth,thepatientexhibitedbradycardia,abnormalEEG(centralapneaspresent)andearlymyoclonicencephalopathy.Thepatient"sepilepsywascharacterizedfurtherby:notederraticseizures(1to5perday),poororalandmotorskills,inabilitytofollowwithgazeandthebrainstemauditoryevokedresponse(BAER)revealedonlywaveformsIandIIIsuggestiveofupperpontinedysfunction.ThedonorwastreatedwithphenobarbitalandKlonopin. | 
| Shippingconditions | Dryice | 
| Storageconditions | Liquidnitrogen | 

		