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Axolbio/Human iPSC-Derived Neural Stem Cells - Alzheimers Disease Patient (PSEN2 N141I)/ax0115/1 Ea
Description:
AxolHumaniPSC-DerivedNeuralStemCells
AxolHumaniPSC-DerivedNeuralStemCells(NSCs)arederivedfromintegration-free,inducedpluripotentstemcells(iPSCs)underfullydefinedneuralinductionconditions.TheNSCsexpresstypicalMarkersofcerebralcorticalneuralstemandProgenitorcellssuchasPAX6,FOXG1andnestin,andspontaneouslyformpolarizedneuraltube-likerosettestructureswhenplatedasamonolayerinculture(seebelow).Additionally,AxolNSCsarecapableofgeneratingaspectrumofcerebralcorticalexcitatoryandinhibitoryneuronsthatareelectricallyactiveandhavetheABIlitytoformfunctionalsynapsesandcircuitsinvitro.Afterthawingandplating,theneuralstemcellsterminallydifferentiatetoacquirematureelectrophysiologicalproperties,andformfunctionalsynapticnetworksoveraperiodof40~50days.
AxolNSCsareeasytodifferentiatetoneuronsormixedneuralcelltypes,followingourprotocolsandusingourtailoredmediaandreagentbundles.AhighlypurepopulationofneuronscanbegeneratedfromAxolNSCsfollowingthesynchronousdifferentiationprotocol.Usingourspecializedcoatingreagents,neuronsderivedfromAxolNSCscanbemaintainedinculturelong-term(>1year).NSCsareavailablefrommultipledonorstosuityourresearchneedsandhavebeencharacterizedextensively.

ProductSpecification:
| Startingmaterial | Dermalfibroblast |
| Donorgender | Female |
| Donorageatsampling | 81yrs |
| HLASEROtype | A2A2,B62(Bw6)B51(Bw4),Cw1Cw2 |
| Karyotype | Normal |
| Reprogrammingmethod | Episomalvector |
| Inductionmethod | Monolayer&chemicallydefinedmedium |
| Size | ≥1.5millioncells |
| Kitcomponents | 1vialofNeuralStemCells(≥1.5millioncells)and1bottleofNeuralPlating-XFMedium(30mL) |
| Celltype | iPSC-derivedneuralstemcells |
| Growthproperties | Adherent |
| Mutation | Presenilin2(PSEN2)N141I |
| Diseaseinformation | Thedonor(Caucasian)exhibitedprogressivedementiaandwasclinicallyaffectedwithAlzheimer"sDisease.Thedonor"spaternalgrandfather,aunt,twocousinsandtwosIBLingswerealsoaffectedwithAlzheimer"sDisease. |
| Shippingconditions | Dryice |
| Storageconditions | Liquidnitrogen |

