Description:
AxolHumaniPSC-DerivedNeuralStemCells
AxolHumaniPSC-DerivedNeuralStemCells(NSCs)arederivedfromintegration-free,inducedpluripotentstemcells(iPSCs)underfullydefinedneuralinductionconditions.TheNSCsexpresstypicalMarkersofcerebralcorticalneuralstemandProgenitorcellssuchasPAX6,FOXG1andnestin,andspontaneouslyformpolarizedneuraltube-likerosettestructureswhenplatedasamonolayerinculture(seebelow).Additionally,AxolNSCsarecapableofgeneratingaspectrumofcerebralcorticalexcitatoryandinhibitoryneuronsthatareelectricallyactiveandhavetheABIlitytoformfunctionalsynapsesandcircuitsinvitro.Afterthawingandplating,theneuralstemcellsterminallydifferentiatetoacquirematureelectrophysiologicalproperties,andformfunctionalsynapticnetworksoveraperiodof40~50days.
AxolNSCsareeasytodifferentiatetoneuronsormixedneuralcelltypes,followingourprotocolsandusingourtailoredmediaandreagentbundles.AhighlypurepopulationofneuronscanbegeneratedfromAxolNSCsfollowingthesynchronousdifferentiationprotocol.Usingourspecializedcoatingreagents,neuronsderivedfromAxolNSCscanbemaintainedinculturelong-term(>1year).NSCsareavailablefrommultipledonorstosuityourresearchneedsandhavebeencharacterizedextensively.
ProductSpecification:
Startingmaterial | Dermalfibroblast |
Donorgender | Female |
Donorageatsampling | 31yrs |
HLASEROtype | A2A24,B18(Bw6)B44(Bw4),Cw2Cw12 |
Reprogrammingmethod | Episomalvector |
Inductionmethod | Monolayer&chemicallydefinedmedium |
Geneticmodification | None |
Size | ≥1.5millioncells |
Kitcomponents | Kitincludes≥1.5MviablecryopreservediPSC-derivedNeuralStemCells(PSEN-1MutationA246E)and1x30mLNeuralPlating-XFMedium(ax0033).. |
Celltype | iPSC-derivedneuralstemcells |
Growthproperties | Adherent |
Mutation | Presenilin1(PSEN1)A426E |
Diseaseinformation | Thedonor(Caucasian)wasclinicallyaffectedwithAlzheimer"sDisease.Onsetofthediseaseoccurredatage45. |
Shippingconditions | Dryice |
Storageconditions | Liquidnitrogen |