Description:
AxolHumaniPSC-DerivedNeuralStemCells 
AxolHumaniPSC-DerivedNeuralStemCells(NSCs)arederivedfromintegration-free,inducedpluripotentstemcells(iPSCs)underfullydefinedneuralinductionconditions.TheNSCsexpresstypicalMarkersofcerebralcorticalneuralstemandProgenitorcellssuchasPAX6,FOXG1andnestin,andspontaneouslyformpolarizedneuraltube-likerosettestructureswhenplatedasamonolayerinculture(seebelow).Additionally,AxolNSCsarecapableofgeneratingaspectrumofcerebralcorticalexcitatoryandinhibitoryneuronsthatareelectricallyactiveandhavetheABIlitytoformfunctionalsynapsesandcircuitsinvitro.Afterthawingandplating,theneuralstemcellsterminallydifferentiatetoacquirematureelectrophysiologicalproperties,andformfunctionalsynapticnetworksoveraperiodof40~50days.
AxolNSCsareeasytodifferentiatetoneuronsormixedneuralcelltypes,followingourprotocolsandusingourtailoredmediaandreagentbundles.AhighlypurepopulationofneuronscanbegeneratedfromAxolNSCsfollowingthesynchronousdifferentiationprotocol.Usingourspecializedcoatingreagents,neuronsderivedfromAxolNSCscanbemaintainedinculturelong-term(>1year).NSCsareavailablefrommultipledonorstosuityourresearchneedsandhavebeencharacterizedextensively.

ProductSpecification:
| Startingmaterial | Dermalfibroblast | 
| Donorgender | Female | 
| Donorageatsampling | 31yrs | 
| HLASEROtype | A2A24,B18(Bw6)B44(Bw4),Cw2Cw12 | 
| Reprogrammingmethod | Episomalvector | 
| Inductionmethod | Monolayer&chemicallydefinedmedium | 
| Geneticmodification | None | 
| Size | ≥1.5millioncells | 
| Kitcomponents | Kitincludes≥1.5MviablecryopreservediPSC-derivedNeuralStemCells(PSEN-1MutationA246E)and1x30mLNeuralPlating-XFMedium(ax0033).. | 
| Celltype | iPSC-derivedneuralstemcells | 
| Growthproperties | Adherent | 
| Mutation | Presenilin1(PSEN1)A426E | 
| Diseaseinformation | Thedonor(Caucasian)wasclinicallyaffectedwithAlzheimer"sDisease.Onsetofthediseaseoccurredatage45. | 
| Shippingconditions | Dryice | 
| Storageconditions | Liquidnitrogen | 

		