Description:
AxolHumaniPSC-DerivedNeuralStemCells
AxolHumaniPSC-DerivedNeuralStemCells(NSCs)arederivedfromintegration-free,inducedpluripotentstemcells(iPSCs)underfullydefinedneuralinductionconditions.TheNSCsexpresstypicalMarkersofcerebralcorticalneuralstemandProgenitorcellssuchasPAX6,FOXG1andnestin,andspontaneouslyformpolarizedneuraltube-likerosettestructureswhenplatedasamonolayerinculture(seebelow).Additionally,AxolNSCsarecapableofgeneratingaspectrumofcerebralcorticalexcitatoryandinhibitoryneuronsthatareelectricallyactiveandhavetheABIlitytoformfunctionalsynapsesandcircuitsinvitro.Afterthawingandplating,theneuralstemcellsterminallydifferentiatetoacquirematureelectrophysiologicalproperties,andformfunctionalsynapticnetworksoveraperiodof40~50days.
AxolNSCsareeasytodifferentiatetoneuronsormixedneuralcelltypes,followingourprotocolsandusingourtailoredmediaandreagentbundles.AhighlypurepopulationofneuronscanbegeneratedfromAxolNSCsfollowingthesynchronousdifferentiationprotocol.Usingourspecializedcoatingreagents,neuronsderivedfromAxolNSCscanbemaintainedinculturelong-term(>1year).NSCsareavailablefrommultipledonorstosuityourresearchneedsandhavebeencharacterizedextensively.

ProductSpecification:
| Startingmaterial | Dermalfibroblast |
| Donorgender | Male |
| Donorageatsampling | 53yrs |
| HLASEROtype | A1A3,B35(Bw6)B47(Bw4),Cw4Cw6 |
| Reprogrammingmethod | Episomalvector |
| Inductionmethod | Monolayer&chemicallydefinedmedium |
| Geneticmodification | None |
| Size | ≥1.5millioncells |
| Kitcomponents | Kitincludes≥1.5MviablecryopreservediPSC-derivedNeuralStemCells(PSEN-1MutationM146L)and1x30mLNeuralPlating-XFMedium(ax0033). |
| Celltype | iPSC-derivedneuralstemcells |
| Growthproperties | Adherent |
| Mutation | Presenilin1(PSEN1)M146L |
| Diseaseinformation | Thedonor(Caucasian)wasclinicallyaffectedwithAlzheimer"sDisease. |
| Shippingconditions | Dryice |
| Storageconditions | Liquidnitrogen |

