Description:
AxolHumaniPSC-DerivedNeuralStemCells
AxolHumaniPSC-DerivedNeuralStemCells(NSCs)arederivedfromintegration-free,inducedpluripotentstemcells(iPSCs)underfullydefinedneuralinductionconditions.TheNSCsexpresstypicalMarkersofcerebralcorticalneuralstemandProgenitorcellssuchasPAX6,FOXG1andnestin,andspontaneouslyformpolarizedneuraltube-likerosettestructureswhenplatedasamonolayerinculture(seebelow).Additionally,AxolNSCsarecapableofgeneratingaspectrumofcerebralcorticalexcitatoryandinhibitoryneuronsthatareelectricallyactiveandhavetheABIlitytoformfunctionalsynapsesandcircuitsinvitro.Afterthawingandplating,theneuralstemcellsterminallydifferentiatetoacquirematureelectrophysiologicalproperties,andformfunctionalsynapticnetworksoveraperiodof40~50days.
AxolNSCsareeasytodifferentiatetoneuronsormixedneuralcelltypes,followingourprotocolsandusingourtailoredmediaandreagentbundles.AhighlypurepopulationofneuronscanbegeneratedfromAxolNSCsfollowingthesynchronousdifferentiationprotocol.Usingourspecializedcoatingreagents,neuronsderivedfromAxolNSCscanbemaintainedinculturelong-term(>1year).NSCsareavailablefrommultipledonorstosuityourresearchneedsandhavebeencharacterizedextensively.
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ProductSpecification:
Startingmaterial | Dermalfibroblast |
Donorgender | Male |
Donorageatsampling | 53yrs |
HLASEROtype | A1A3,B35(Bw6)B47(Bw4),Cw4Cw6 |
Reprogrammingmethod | Episomalvector |
Inductionmethod | Monolayer&chemicallydefinedmedium |
Geneticmodification | None |
Size | ≥1.5millioncells |
Kitcomponents | Kitincludes≥1.5MviablecryopreservediPSC-derivedNeuralStemCells(PSEN-1MutationM146L)and1x30mLNeuralPlating-XFMedium(ax0033). |
Celltype | iPSC-derivedneuralstemcells |
Growthproperties | Adherent |
Mutation | Presenilin1(PSEN1)M146L |
Diseaseinformation | Thedonor(Caucasian)wasclinicallyaffectedwithAlzheimer"sDisease. |
Shippingconditions | Dryice |
Storageconditions | Liquidnitrogen |