Description:
AxolHumaniPSC-DerivedNeuralStemCells 
AxolHumaniPSC-DerivedNeuralStemCells(NSCs)arederivedfromintegration-free,inducedpluripotentstemcells(iPSCs)underfullydefinedneuralinductionconditions.TheNSCsexpresstypicalMarkersofcerebralcorticalneuralstemandProgenitorcellssuchasPAX6,FOXG1andnestin,andspontaneouslyformpolarizedneuraltube-likerosettestructureswhenplatedasamonolayerinculture(seebelow).Additionally,AxolNSCsarecapableofgeneratingaspectrumofcerebralcorticalexcitatoryandinhibitoryneuronsthatareelectricallyactiveandhavetheABIlitytoformfunctionalsynapsesandcircuitsinvitro.Afterthawingandplating,theneuralstemcellsterminallydifferentiatetoacquirematureelectrophysiologicalproperties,andformfunctionalsynapticnetworksoveraperiodof40~50days.
AxolNSCsareeasytodifferentiatetoneuronsormixedneuralcelltypes,followingourprotocolsandusingourtailoredmediaandreagentbundles.AhighlypurepopulationofneuronscanbegeneratedfromAxolNSCsfollowingthesynchronousdifferentiationprotocol.Usingourspecializedcoatingreagents,neuronsderivedfromAxolNSCscanbemaintainedinculturelong-term(>1year).NSCsareavailablefrommultipledonorstosuityourresearchneedsandhavebeencharacterizedextensively.

ProductSpecification:
| Startingmaterial | Dermalfibroblast | 
| Donorgender | Female | 
| Donorageatsampling | 38yrs | 
| HLASEROtype | A2A2,B62(Bw6)B51(Bw4),Cw1Cw2 | 
| Reprogrammingmethod | Episomalvector | 
| Inductionmethod | Monolayer&chemicallydefinedmedium | 
| Geneticmodification | None | 
| Kitcomponents | 1vialofNeuralStemCells(≥1.5millioncells)and1bottleofNeuralPlating-XFMedium(30mL) | 
| Celltype | iPSC-derivedneuralstemcells | 
| Growthproperties | Adherent | 
| Mutation | Presenilin1(PSEN1)L286V | 
| Diseaseinformation | Thedonor(Caucasian)wasclinicallyaffectedwithAlzheimer"sDisease.Onsetwasatage39. | 
| Concentration | 1.5millionviablecells | 
| Shippingconditions | Dryice | 
| Storageconditions | Liquidnitrogen | 

		